Microscopy Lab

PowerPoint from introduction of lab

The text of the assignment is here.  You can copy and edit this Word document to avoid retyping the questions- be sure to add add your name and the date!

Fun Links

Microscope

http://www.edumedia-sciences.com/a301_l2-microscope.html

Parts of Microscope

http://www.wisc.edu/ansci_repro/lab/lab3/animations/microscopea.swf

http://www.biology.ualberta.ca/facilities/multimedia/uploads/intro-biology/microscope.swf

http://science.nhmccd.edu/Biol/dropdrag/microscope.htm

http://science.nhmccd.edu/Biol/dropdrag/microscope2.htm

Other links

  • Virtual Library of Microscopy- loads of links
  • Dennis Kunkel's Microscopy- some of the most spectacular SEM micrographs on the Web.  He has nice, succinct, descriptions of scanning and transmission electron microscopy.  Just the right information for your lab assignment.  See if you can find it...
  • Cells Alive  Great site- lots of wonderful biological images. "Enhancing the microscope image" in the table of contents will lead you to some good descriptions of various types of light microscopes.
  • Molecular expressions  Optical microscopy pages at Florida State University.  This is an amazing site- loads of information and pictures.  There are some great Java applets to explain how lenses work.  Be selective in what you read- this site is very detailed.  Here is an excerpt to show you what I mean:
Excerpt from the "Molecular Expressions" site:

"The second most convenient method for photographing beer is to atomize or convert the beer into a fine mist and to blast or spray the mist onto a smooth hydrophobic surface such as silicon, germanium, polystyrene, or treated glass. The photomicrograph in Figure 2 exhibits this technique when applied to the same Budweiser sample used to produce Figure 1. In this case the beer was chilled to 4° C, placed in a container and pressurized with Freon before being atomized and sprayed directly onto the surface of a highly polished silicon wafer.

The sample was imaged in a reflected light differential interference contrast (DIC) microscope using a quartz compensating plate between the Wollaston prisms and polarizers. DIC microscopy is a rather complicated technique, but it can be used effectively to produce dramatic and colorful images of both liquid and crystalline samples. The major drawback to DIC microscopy is the high cost of the equipment, which will generally add about $10,000.00 to the price of a polarized light microscope."

Figure 2. DIC photomicrograph of chilled Budweiser beer
sprayed onto a highly polished silicon surface and imaged
with a retardation plate.

  • Nikon Microscopy U- powered by Molecular Expressions.  Micro-movies!
  • I mentioned that microscopes were popular entertainment in Victorian times.  Those times continue to the present.   Micscape magazine is an excellent source for amateur stuff.  Microscopy UK is the home of amateur microscopy on the web.  Here is the page of the Quekett Microscopical Club-a venerable and very British microscopy club.
  • An Introduction to Microscopy by Wim. van Egmond an excellent "how to" for the hobbyist, associated with Micscape. 


Back to BIO 121 Lab Home Page